HAP1细胞库

产品介绍

一个细胞背景，多种细胞应用；

拥有7500多基因敲除的细胞模型中可供学术界、生物技术和制药研究实验室信任和选择；

发表于Nature，Journal of Cell Science, Cell Reports等一系列同行评审文献支持。

产品特点

HAP1细胞系是单倍体基因型，每一个基因都只有一个拷贝，确保编辑后的等位基因不会被其他等位基因掩盖，所以HAP1细胞是基因编辑模型的一个很好的选择。

1）HAP1细胞的特征：

2）在被稀释成单细胞的条件下仍可维持生长

3）易于转染

4）快速的倍增时间

5）HAP1是来源于男性慢性粒细胞白血病（CML）细胞株KBM-7的人近单倍体细胞株。

应用领域

Horizon的HAP1细胞系已扩展到多种研究应用领域，如病毒学、线粒体、凋亡/自噬、基因组完整性/功能基因组学、药物验证等。

（1）病毒学领域：研究人员经常分析人类HAP1细胞感染过程中相关基因的功能作用。宿主基因的失活可导致KO的生存能力提高（与WT细胞相比），这表明它们对于有效的病毒复制至关重要。实验结果通常基于细胞生存能力、报告病毒¹、病毒定量²和病毒复制周期³、斑块直径/大小测定⁴或干扰素测量等检测指标^5,6；

（2）在凋亡/自噬相关研究中，混合HAP1突变筛选实验通过标记细胞特异性中间产物，随后进行FACS细胞分类和HAP1 KO验证，从而识别生物合成途径中的负调节因子¹³。此外，HAP1细胞可通过自噬标记物的免疫染色，用于表征自噬过程中的WT基因¹⁴；

（3）在药物功能验证研究中，在WT-和KO-HAP1细胞系中进行癌症相关抑制剂的不同剂量-反应曲线，可以提供由某些基因产物驱动的抑制剂的细胞毒性的证据¹⁵；

（4）在基因组完整性/功能基因组学领域，与WT对照细胞相比，HAP1-KOs的染色体断裂可以阐明参与DNA修复机制的关键基因¹⁶。

点击如下链接，查看不同领域，应用Horizon的HAP1细胞株发表文献。

https://horizondiscovery.com/-/media/Files/Horizon/resources/Reading-lists/hap1-ref-reading.pdf

细胞查找

点击如下链接，查询相应的HAP1细胞株。

https://horizondiscovery.com/en/engineered-cell-lines/products/human-hap1-knockout-cell-lines

规格参数

想知道是否感兴趣的基因已经有构建好的HAP1基因敲除细胞株，点击如下链接，输入基因名称，便可查询相关信息

https://horizondiscovery.com/products/gene-editing/cell-line-models/PIFs/Human-HAP1-Knockout-Cell-Lines

如需订购，请记录货号，联系我们。也可直接联系我们查询相关基因敲除的细胞株。

参考文献

使用HorizonDiscovery的HAP1突变细胞株，每年有大量文献发表。可参考如下部分精选或者文末下载文献汇总

部分已发表文献

[1] Flint M , Chatterjee P , Lin D L , et al. A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus[J]. Nature Communications, 2019, 10(1).

[2] Lyoo H , Van D , Dorobantu C M , et al. ACBD3 Is an Essential Pan-enterovirus Host Factor That Mediates the Interaction between Viral 3A Protein and Cellular Protein PI4KB[J]. Mbio, 2019, 10(1).

[3] Moskovskich A , Goldmann U , Kartnig F , et al. The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection[J]. Scientific Reports.

[4] Lafontaine E , Miller C M , Permaul N , et al. Ribosomal protein RACK1 facilitates efficient translation of poliovirus and other viral IRESs. 2019.

[5] Pokharel S M , Shil N K , Gc J B , et al. Integrin activation by the lipid molecule 25-hydroxycholesterol induces a proinflammatory response[J]. Nature Communications, 2019, 10(1).

[6] Shen Q , Mathew T , Mahadevan K , e

部分高分文献摘选

2019

• A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus Nature Communications
• Integrin activation by the lipid molecule 25-hydroxycholesterol induces a proinflammatory response Nature Communications
• A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA[Ser]Sec Gene Cells
• Bypassing pan-enterovirus host factor PLA2G16 Nature Communications
• iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture; Nature Comms
• PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression; Nature Comms
• Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes; PNAS
• A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus; Nature Comms
• Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells; Autophagy

2018

• Transcriptional and epigenetic profiling of nutrient-deprived cells to identify novel regulators of autophagy; Autophagy
• Accurate classification of BRCA1 variants with saturation genome editing; Nature
• The Importance of Poly(ADP-Ribose) Polymerase as a Sensor of Unligated Okazaki Fragments during DNA Replication; Molecular Cell
• Validating the concept of mutational signatures with isogenic cell models; Nature Comms
• Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48; Nature Comms

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