PEI转染试剂 MW25000(PEI 25K)

PEI转染试剂 MW25000(PEI 25K) 聚乙烯亚胺

Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K)

Polysciences 转染试剂PEI 25K产品简介

Polysciences 转染试剂系列是基于聚乙烯亚胺（Polyethylenimine，PEI）的产品，因其较高的转染效果，已应用于工业化生产。聚乙烯亚胺是一种具有较高的阳离子电荷密度的有机大分子，每相隔二个碳原子，即每“第三个原子都是质子化的氨基氮原子，使得聚合物网络在各pH下能充当有效的“质子海绵”体(proton sponge)。PEI可用作转染试剂，它可以缩聚DNA分子形成颗粒并转染真核细胞。有实验证明，分子量为25000的线性PEI即Polysciences 23966（PEI 25K）转染效率表现优良。

PEI 25K（25kDa 线性聚乙烯亚胺）是一款性能强劲、广受信赖且经济的瞬时转染试剂。在 HEK293 和 CHO 表达系统中，该试剂可在从 96 孔板到 100L 生物反应器的全规模范围内，实现稳定一致的高基因表达水平。

每年都有越来越多的科研人员和企业选择Kyfora Bio的 PEI 产品，以在研发工作中获得关键竞争优势。与市场上大多数同类转染试剂相比，采用 PEI 自行配制转染工作液，可将整体转染成本降低高达 40%

转染试剂产品特点

1、适用于生产 mABs、rAbs、bsABs和病毒载体(AVV、LV、BEV)

2、在HEK293、CHO 和 Sf9 细胞系中高度有效

3、适用于从研发到工业化生产的各个阶段

4、可预测，可大规模生产

5、两周内即可生成大量目标蛋白或病毒

6、高转染效率

7、多个已发表的文献支持

更多内容可查看： 为什么全球数万篇文献都选择Kyfora by Polysciences的PEI转染试剂？

部分相关引用文献：

1. Wang, M., Wen, X., ..., Lu, & T. (2026). RNF207 promotes colorectal cancer growth by regulating the Hippo-YAP pathway via enhanced MST1 ubiquitination and degradation.. *Journal of gastroenterology*. https://doi.org/10.1007/s00535-026-02440-2

2. Li, P., Liu, L., ..., Fan, & H. (2026). STUB1-mediated ubiquitination regulates Fli-1 stability and CD4⁺T cell activation during inflammation.. *Molecular medicine (Cambridge, Mass.)*. https://doi.org/10.1186/s10020-026-01494-5

3. Xiang, M., Constance L., & C. (2026). Combined Disruption of Multiple Cytokine Signaling Pathways Enables One-Step Anterograde Tracing with Vesicular Stomatitis Virus. *bioRxiv*. https://doi.org/10.64898/2026.05.22.727255

4. Lim, J., Oh, S., ..., Sarah Shin, & O. (2026). Microglial MARCO facilitates Varicella zoster virus uptake and triggers TLR2-mediated neuroinflammation. *Journal of Biomedical Science*. https://doi.org/10.1186/s12929-026-01256-9

5. Josue M. J., R., Ariadna, P., ..., Arnim, & P. (2026). AMPK-dependent FLCN phosphorylation activates a survival response to hypoxia. *bioRxiv*. https://doi.org/10.64898/2026.05.20.726614

6. Jin, R., Zhong, Z., ..., Wang, & Y. (2026). LATS kinase activity and tumor suppressor function are regulated by a second autophosphorylation site.. *The Journal of Biological Chemistry*. https://doi.org/10.1016/j.jbc.2026.113177

7. Liang, W., J Mueller, S., ..., E Sanjana, & N. (2026). Essential lncRNAs in the human transcriptome.. *Cell Genomics*. https://doi.org/10.1016/j.xgen.2026.101253

8. Majid, J., Hongyan, Z., ..., Jian, & H. (2026). An N-terminal amphipathic helix governs activity and conformational dynamics of Nramp metal transporters. *bioRxiv*. https://doi.org/10.64898/2026.05.17.725737

9. Emma, J., Helena, A., ..., Kirsty J., & M. (2026). Global identification of neuronal and astrocytic integral membrane proteins that require Retromer for their endosomal recycling. *bioRxiv*. https://doi.org/10.64898/2026.05.14.724903

10. Chu, J., Zhao, Q., ..., Kumar Gara, & S. (2026). CDK1 Phosphorylates KAT8 at Ser348 to Stabilize the MSL Complex and Promote H4K16 Acetylation in Non-Small Cell Lung Cancer. *Cells*. https://doi.org/10.3390/cells15100897

11. Samy, O., Pietro Erminia, D., ..., Catherine, & A. (2026). Clinically relevant AAV8- PEX1 gene therapy preserves retinal integrity and function long-term in a murine model of Zellweger spectrum disorder. *bioRxiv*. 

12. Choi, H., Kim, S., ..., Baek, & K. (2026). Ubiquitin-Specific Protease 49 Interacts with Bax to Modulate Apoptosis.. *International journal of molecular sciences*. https://doi.org/10.3390/ijms27094102

13. Cheng, L., Chen, C., ..., Hu, & Z. (2026). Targeting cancer-associated cell surface RNAs with oligonucleotide-drug conjugates enables broad antitumor activity. *bioRxiv*. https://doi.org/10.64898/2026.05.08.723688

14. Afrim, A., Martina, A., ..., Isabel, & D. (2026). Self-organized hemanoids derived from human iPSCs create a niche that produces definitive extraembryonic hematopoiesis. *bioRxiv*. https://doi.org/10.64898/2026.05.05.722134

15. Garcia Freitas-Filho, E., Zaidan, I., ..., Dias Cunha, & L. (2026). RAB5c orchestrates LC3-associated phagocytosis to promote microbicidal function of macrophages. *Science Advances*. https://doi.org/10.1126/sciadv.adz0196

PEI 25K粉末配置方法

1）将1g PEI 25K放入盛有900 mL水的玻璃烧杯中；

2）放入搅拌转子，放置于磁力搅拌器上，设置搅拌程序以形成一个较小的漩涡；

3）加入盐酸滴定至pH<2.0；

4）盖上烧杯敞口，搅拌3小时，直到粉末完全溶解；

5）加入1N氢氧化钠，直至pH达到 6.3~6.7。每次加入一点，待充分混合后再检查pH值。注意：PEI 25K的配制最高可兼容的pH为7.1；

6）将溶液转移到1L玻璃量筒中，加入水定容至1L；

7）用无菌真空过滤膜过滤配制的转染试剂溶液；

8）按需分装，-20°C储存；

注：未经配制的粉末有效期为2年。

PEI 25K易溶于热水，低pH值的冷水，甲醇和乙醇等，不溶于苯，乙醚和丙酮等。

配置成液体后分装在合适的瓶子中，可在-20℃保存一年。解冻之后可在4℃保存，建议2周之内尽快用完。分装试剂不能反复冻融。